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As an eventful year draws to a close, it is always a reason to look back and look forward. This year the Core Facility has acquired a brand new technology with our two new Sony ID7000 spectral analyzers. This technology provides the opportunity to greatly expand flow cytometric panels. With good panel design and some…
The 7 laser configuration gives you increased panel design flexibility and more sensitivity due to less data spread after unmixing. For more details, read following technical note: Using the 320-nm Laser on the ID7000™
In 2018 the Flow Cytometry Core Facility was able to acquire five new instruments.
A FACSAria Fusion cell sorter, a second LSRFortessa cell analyzer and an ImageStream image cytometer were granted by the DFG through a large device application. (“Forschungsgroßgeräte” nach Art.91b GG)
Special thanks goes to the excellence cluster “ImmunoSensation”and to the SFB TR57 . A FACSMelody cell sorter and a Attune NXT flow cytometer could be purchased by their help. Furthermore, we could upgrade the Image Stream shortly after it was delivered, since we found out, that one of the planned experiments would require up to 20 hours of measurement time. This is definitely a job for an autosampler.
What is spectral flow cytometry? In contrast with conventional flow cytometry that detects the emission peak of fluorochromes by the use of optical filters, spectral flow cytometry distinguishes the shapes of emission spectra along a large range of continuous wave lengths. The data is analyzed with an algorithm that replaces compensation matrices and treats auto-fluorescence…