Allgemein / Announcement / Core Facilities / Education / Flow Cytometry / Technics
by andolf · Published December 10, 2021
Abstract The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine...
We are happy to announce, that our move to the new laboratory in the BMZII was successful. Access to the building for external users is still a little bit tricky, though. Our facility is...
Dear Users, since 2020 is coming to an end we´ld like to thank you for your trust and understanding during those special times. We hope that our restrictions didn´t hinder your measurements too much,...
Dear users of the Flow Cytometry Core Facility, first of all, we wanted to thank all our users for accepting and respecting the rules and regulations established to deal with the corona pandemic as...
Apple’s latest macOS (version 10.15 Catalina) was released this week. There are two issues relating to FlowJo we are aware of: On Catalina, dongles are not authenticating FlowJo v10 when launched from the...
As most of our users might already know, Natalio, Peter and I (Andreas) are three of over 300 coauthors of the “Guidelines for the use of flow cytometry and cell sorting in immunological studies”...
At this years 34th congress of the international society for advancement of cytometry (Cyto) in Vancouver we proudly presented our work „Visualizing Data Spreading after Compensation as a Decision-making Tool for Multicolor Panel Design...
Core Facilities / Flow Cytometry / Technics
by andolf · Published February 19, 2019 · Last modified February 20, 2019
Phagosome maturation after bacterial uptake: Dr. Katarzyna Jobin,Scientist (Kurts Lab, Institute of experimental Immunology): In this example phagosomes and lysosomes where stained in different colors. If both are colocalized in the cell, the phagosomes...
Announcement / Core Facilities / Flow Cytometry / Technics
by andolf · Published February 19, 2019 · Last modified March 6, 2019
In 2018 the Flow Cytometry Core Facility was able to acquire five new instruments.
A FACSAria Fusion cell sorter, a second LSRFortessa cell analyzer and an ImageStream image cytometer were granted by the DFG through a large device application. (“Forschungsgroßgeräte” nach Art.91b GG)
Special thanks goes to the excellence cluster “ImmunoSensation”and to the SFB TR57 . A FACSMelody cell sorter and a Attune NXT flow cytometer could be purchased by their help. Furthermore, we could upgrade the Image Stream shortly after it was delivered, since we found out, that one of the planned experiments would require up to 20 hours of measurement time. This is definitely a job for an autosampler.
What is spectral flow cytometry? In contrast with conventional flow cytometry that detects the emission peak of fluorochromes by the use of optical filters, spectral flow cytometry distinguishes the shapes of emission spectra along...
Cytometry A, Journal