FCCF Corona Measures (November Update)
Dear users of the Flow Cytometry Core Facility, first of all, we wanted to thank all our users for accepting and respecting the rules and regulations established to deal with the corona pandemic as...
FlowJo and macOS Catalina (v10.15)
Apple’s latest macOS (version 10.15 Catalina) was released this week. There are two issues relating to FlowJo we are aware of: On Catalina, dongles are not authenticating FlowJo v10 when launched from the...
Updated Version of the Flow Guidelines, published in the European Journal of Immunology, is now online!
As most of our users might already know, Natalio, Peter and I (Andreas) are three of over 300 coauthors of the “Guidelines for the use of flow cytometry and cell sorting in immunological studies”...
FCCF @ Cyto 2019
At this years 34th congress of the international society for advancement of cytometry (Cyto) in Vancouver we proudly presented our work „Visualizing Data Spreading after Compensation as a Decision-making Tool for Multicolor Panel Design...
Snapshots of the first experiments run on our new image cytometer
Phagosome maturation after bacterial uptake: Dr. Katarzyna Jobin,Scientist (Kurts Lab, Institute of experimental Immunology): In this example phagosomes and lysosomes where stained in different colors. If both are colocalized in the cell, the phagosomes...
A comprehensive overview over all new devices of the Flow Cytometry Core Facility.
In 2018 the Flow Cytometry Core Facility was able to acquire five new instruments.
A FACSAria Fusion cell sorter, a second LSRFortessa cell analyzer and an ImageStream image cytometer were granted by the DFG through a large device application. (“Forschungsgroßgeräte” nach Art.91b GG)
Special thanks goes to the excellence cluster “ImmunoSensation”and to the SFB TR57 . A FACSMelody cell sorter and a Attune NXT flow cytometer could be purchased by their help. Furthermore, we could upgrade the Image Stream shortly after it was delivered, since we found out, that one of the planned experiments would require up to 20 hours of measurement time. This is definitely a job for an autosampler.
Seminar: Full-Spectrum Flow Cytometry 15th January 2019, 10:00-12:00
What is spectral flow cytometry? In contrast with conventional flow cytometry that detects the emission peak of fluorochromes by the use of optical filters, spectral flow cytometry distinguishes the shapes of emission spectra along...
Melody cell sorter and second Fortessa are now ready to use.
The BD FACSMelody is a small and easy to use cell sorter, equipped with three lasers (405nm, 488nm, 561nm) and capable of detecting 11 parameters. This instrument is intended to be used without the assistance of a specialized operator. With the newly designed BD FACSChorus™ software, researchers are guided throughout the entire cell sorting process using advanced automation technology. It is designed to eliminate manual steps and has a really simplified workflow….
The BD LSRFortessa analyzer was acquired in order to eliminate the capacity shortages of the existing Fortessa. The two instruments have an identical configuration to facilitate the transfer of your experiments from one machine to another…
New technology available in the Flow Cytometry Core Facility
Many of you will already know that the FCCF could expand its equipment park considerably this year. By now, all instruments are delivered, but not all of them are ready to use, due to...
Flow Cytometric Analysis of Proliferation and Apoptosis
WHEN: 8. November 2018 @ 10:00 – 16:00 WHERE: Hörsaal, klein BMZ Gebäude 406 Sigmund-Freud-Straße 25, 53127 Bonn Germany Agenda: + 10:00 Introduction Flow Cytometry + 10:30 Apoptosis & Proliferation – Old Tricks And...
