We would very much appreciate it if you could complete the following survey:

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We are pleased to inform you that we have received approval of our application for a new high-end spectral cell sorter. We would like to learn more about the experience of our users with our cell sorting services in the Flow Cytometry Core Facility and about your current needs and aspirations for the future. Since we are in the fortunate position of being able to purchase a new cell sorter in the near future, your help will be very useful in deciding which of the available sorters to choose.

General questions:

How often have you used the Core Facility cell sorting services in the last 12 months? (approximately)
Overall, how satisfied were you with the core facility service?
How satisfied were you with the cell sorting results?
1 - poor ... 5 - excellent
1 - poor ... 5 - excellent
1 - poor ... 5 - excellent
1 - poor ... 5 - excellent

Biological questions:

Which types of cells are mostly sorted?
Which types of cells are mostly sorted?
Do you sort untested human material or patient samples?
What kind of downstream applications do you mainly have?
Do you have any survival issues after sorting?

Technical questions:

What nozzle sizes do you normally use?
Which collection devices do you normally use?
How many populations do you primarily sort?
Would you like to sort more than four populations?
How many fluorochromes do you currently use in your sorting experiments?
Would you like to stain more, if possible?
Would you like to use your spectral panels for cell sorting?
Do you think you would benefit from images in your sorting experiments?

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A comprehensive overview over all new devices of the Flow Cytometry Core Facility.
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A comprehensive overview over all new devices of the Flow Cytometry Core Facility.

Byandolf

In 2018 the Flow Cytometry Core Facility was able to acquire five new instruments.

A FACSAria Fusion cell sorter, a second LSRFortessa cell analyzer and an ImageStream  image cytometer were granted by the DFG through a large device application. (“Forschungsgroßgeräte” nach Art.91b GG)

Special thanks goes to the excellence cluster “ImmunoSensation”and to the SFB TR57 .  A FACSMelody cell sorter and a Attune NXT flow cytometer could be purchased by their help. Furthermore, we could upgrade the Image Stream shortly after it was delivered, since we found out, that one of the planned experiments would require up to 20 hours of measurement time. This is definitely a job for an autosampler.

Melody cell sorter and second Fortessa are now ready to use.
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Melody cell sorter and second Fortessa are now ready to use.

Byandolf

The BD FACSMelody is a small and easy to use cell sorter, equipped with three lasers (405nm, 488nm, 561nm) and capable of detecting 11 parameters. This instrument is intended to be used without the assistance of a specialized operator. With the newly designed BD FACSChorus™ software, researchers are guided throughout the entire cell sorting process using advanced automation technology. It is designed to eliminate manual steps and has a really simplified workflow….

The BD LSRFortessa analyzer was acquired in order to eliminate the capacity shortages  of the existing Fortessa. The two  instruments have an identical configuration to facilitate the transfer of your experiments from one machine to another…