Instrumentation

Cellsorter:

Cell sorting is mainly performed by experienced operators from the Core Facility. Three high-end, high-speed cell sorters are available:
BD FACSAria Fusion (biosafety level S2 approval), BD FACSAria III, BD Influx
For operator free cell sorting of less advanced applications the BD FACSMelody system is available.

BD FACS Aria Fusion BD FACS Aria III BD Influx BD FACSMelody

Project number DFG:

387333827

Project number DFG:

216372545

   

Analyzer:

Researchers are trained by Core Facility personnel to perform their experiments without assistance of an operator. The Core Facility takes care of seven analyzers from standard 3 laser, eight color systems to five laser, 18 color instruments. Users receive detailed advice and support on the choice of dyes and equipment that are best suited to answer their scientific questions.
Systems available include:
BD LSRFortessa (2X), FACSCantoII (3X), Miltenyi MACSQuant

BD FACSCanto
Canto I Canto II Canto III
 
BD LSRFortessa SORP Miltenyi MACSQuant X
Fortessa I Fortessa II    

Project number DFG:

216372401

Project number DFG:

387335189

   

Spectral Cytometry:

Spectral Cytometry acquires always the whole spectrum of the emitted light for each excitation laser and uses sophisticated unmixing algorithms to separate the signals from each fluorochrome. The advantages of this technology are, that scientists can combine way more fluorochromes in the same panel even if their emission spectra highly overlap. With our current 5-laser configuration, it is capable to analyze up to 30 colors or more. Furthermore, cell-intrinsic autofluorescence can be treated as a color and unmixed as well.

 

 

 

 

 

 

Imaging Cytometry:

Imaging Flow Cytometry combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. The Amnis ImageStream X MarkII imaging cytometer is fully equipped with three objectives and seven excitation lasers.
Researchers receive support in experiment design and data analysis by the Core Facility staff.

ImageStream X MarkII

Project number DFG:

389568007

Bead Based Cytometry:

Multiplexed, bead-based analysis of cytokines, hormones or many other soluble bio-molecules can be conducted on a Flow Cytometer specially developed for this application: Luminex FlexMAP 3D

 
FlexMap 3D