As most of our users might already know,  Natalio, Peter and I (Andreas) are three of over 300 coauthors of the “Guidelines for the use of flow cytometry and cell sorting in immunological studies” published in the European Journal of Immunology. The Guidelines are a comprehensive collection of methods, protocols and informations about any kind of flow cytometric application, written under the leadership of Andreas Radbruch and Andrea Cossarizza.

Since 2017 it was cited over 200 times (google scholar) and it became one of the most popular resources available for free and online.

Now, only two years after the initial publication the second, revised edition was published in the October issue of the EJI.


These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.


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