What is spectral flow cytometry?

In contrast with conventional flow cytometry that detects the emission peak of fluorochromes by the use of optical filters, spectral flow cytometry distinguishes the shapes of emission spectra along a large range of continuous wave lengths.

The data is analyzed with an algorithm that replaces compensation matrices and treats auto-fluorescence as an independent parameter. Thus, spectral flow cytometry is capable to discriminate fluorochromes with similar emission peaks and provide multi-parametric analysis without conventional compensation requirements.

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