A comprehensive overview over all new devices of the Flow Cytometry Core Facility.
In 2018 the Flow Cytometry Core Facility was able to acquire five new instruments.
A FACSAria Fusion cell sorter, a second LSRFortessa cell analyzer and an ImageStream image cytometer were granted by the DFG through a large device application. (“Forschungsgroßgeräte” nach Art.91b GG)
Special thanks goes to the excellence cluster “ImmunoSensation”and to the SFB TR57 . A FACSMelody cell sorter and a Attune NXT flow cytometer could be purchased by their help. Furthermore, we could upgrade the Image Stream shortly after it was delivered, since we found out, that one of the planned experiments would require up to 20 hours of measurement time. This is definitely a job for an autosampler.
Seminar: Full-Spectrum Flow Cytometry 15th January 2019, 10:00-12:00
What is spectral flow cytometry? In contrast with conventional flow cytometry that detects the emission peak of fluorochromes by the use of optical filters, spectral flow cytometry distinguishes the shapes of emission spectra along a large range of continuous wave lengths. The data is analyzed with an algorithm that replaces compensation matrices and treats auto-fluorescence…
Melody cell sorter and second Fortessa are now ready to use.
The BD FACSMelody is a small and easy to use cell sorter, equipped with three lasers (405nm, 488nm, 561nm) and capable of detecting 11 parameters. This instrument is intended to be used without the assistance of a specialized operator. With the newly designed BD FACSChorus™ software, researchers are guided throughout the entire cell sorting process using advanced automation technology. It is designed to eliminate manual steps and has a really simplified workflow….
The BD LSRFortessa analyzer was acquired in order to eliminate the capacity shortages of the existing Fortessa. The two instruments have an identical configuration to facilitate the transfer of your experiments from one machine to another…
New technology available in the Flow Cytometry Core Facility
Many of you will already know that the FCCF could expand its equipment park considerably this year. By now, all instruments are delivered, but not all of them are ready to use, due to different reasons. The first instrument we wanted to introduce to you, is the ImageStream®X MarkII by Amnis®. This cytometer offers our…
FLOW CYTOMETRY COURSE BONN 19.October.2017 Seminarraum 5, 1OG, Lehrgebäude 9:30-13:00
Seminarraum 5, 1OG, Lehrgebäude 9:30-13:00 9:30-10:00 Introduction to Flow Cytometry 10:15-11:30 Panel Design & Experimental Setup Optimization Strategies Example 8-color Panel Optimization 11:45-12:15 New Technologies for Flow Cytometry PrimeFlow RNA TM Detection Assay SuperBright TM polymer dyes We are looking forward meeting you Drinks and snacks will be available Viktoria Reckert…
Flow Cytometry Day 2017
Tuesday, August 1th 2017, 10:00am Lehrgebäude Hörsaal Flow Cytometry is in some way not a classical hard science. There are sometimes several ways to get the answer for the question you asked. However there are some rules that are good to follow. It is in some way like cooking, there a lot of recipes out…
New management of the Flow Cytomety Core Facility
As some of you might already know that structural changes have taken place at the Flow Cytometry Core Facility during the last months. Dr. Elmar Endl, who established the Core Facility in 2004 with great success, took over a new position in a faculty committee (Dekanatskomission Core Facilities) as Core Facilities Business and Operations Coordinator….
LUNARIS™ a New Technology for Multiplex Cytokine Detection with only 3µl sample volume
AYOXXA’s new multiplex protein detection platform, called LUNARIS™ is now available in the core facility. With ready-to-use BioChips and the respective Analysis Software, LUNARIS™ enables to quantify multiple protein targets in a single sample, particularly in areas where precious sample volume is a limiting factor. YOUR ADVANTAGES: measure multiple protein biomarkers in down to 3…
We will move our servers and sorry, but you have to move too
Not that long ago in 2010 we moved our IT infrastrucuture to new hardware, to be prepared for future requirements. The future has come and is gone by now. So once again we have to move on. The next weeks will be exciting, both for us and you. We first will shutdown our old online…