Invited speaker: Dr. Thomas Bauer
Preliminary program:
10.00 – 10.30 Introduction: Flow Cytometry Core Facility Bonn by Andreas Dolf
10.30 – 11.15 Flow Cytometry: Principles, Instrument Set up, Optics, Filter, Staining
11.15 – 11.30 Break
11.30 – 12.15 Multicolor Flow Cytometry; Fluorescent Dyes, their properties, selection, sensitivities
12.15 – 13.00 Break
13.00 – 14.00 How to optimize Flow Cytometry data acquisition and experimental design to receive optimal results
14.00 – 14.15 Break
14.15 – 15.00 Apoptosis detection and functional assays: Viability, Vitality, Proliferation….
15.00 – 15.30 Multiplex Cytokine Detection
15.30 – 16.00 Data analysis tools to handle multidimensional data
———-
| 10:00am – 12:00am | a basic introduction into flow cytometry |
| topics | -instruments
-reagents -controls |
Topics:
· Load samples into the workspace
· Analyze the control sample in detail
· Copy gates and statistics to all samples
· Verify gates on all samples
·Generate a graphical report
·Generate a table of statistics
· Batch analysis
Hands-on:
Use your own computer with a working Flowjo v.10 installation for a hands-on session.
There is a 30-day-trail-version available on FlowJo.com.
Presenter:
Maximilian Germer (Flow Cytometry Core Facility)
Registration required to get zoom link.
