Olympus IX81 widefield microscope

Location: Building 82, UG1 056

Technical Information:

The Olympus IX81 is a widefield fluorecence microscope. However, the attached STEDYCON unit also offers super-resolution STED imaging and conventional confocal imaging.

Objectives

MagnificationTypeNAPhcvs (mm)WDImmersion
(2.5xPlanNeofluar0.0750.179.5dry)
4xPlanN UIS20.1 18.5dry
(10xUPlanFL0.31 10dry)
20xLCPlanFL0.41 6.75dry
40xUPlanApo0.850.11 – 0.230.2dry
60xPlanApo1.40.17 oil
100xUPlanSApo1.40.170.13oil
       

Filters

DAPI, CFP, YFP, GFP, Cy3, TxRed, Cy5

Camera

Hamamatsu Orca Flash 4.0 LT+ (C11440-42U30)

Light source

Olympus MT20 (Xenon arc burner)

Accessories

Abberior STEDYCON super-resolution microscope

Software

Olympus CellR software