Zeiss LSM980 with Airyscan 2 confocal microscope

Location: NER, UG1.056a

Technical information:

Objectives

MagnificationTypeWD (mm)Cvs (mm)NAImmersion
2.5XEC Plan-NEOFLUAR (420320-9902-000)8.80.170.085dry
10XPApo (420640-9900-000)2.00.170.45dry
20XPApo (420650-9903-000)0.550.170.8dry
40XLD LCI PApo FCS (420862-9970-713)0.40.15 – 0.191.2 corrwater
63XC PApo DIC UV-IR (421782-9900-799)0.140.171.40oil

Laser lines

405 nm

445 nm

488 nm

514 nm

561 nm

639 nm

Accessories

Spectral detector (32 GaAsP + 2 MA-PMT)

Airyscan 2 detector (32 GaAsP)

Transmitted light detector (T-PMT 2)

Axiocam 807 mono

dark environmental chamber

CO2-incubation

AI Sample Finder

Definite Focus 3

Software

Zeiss ZEN blue 3.11

Modules: Airyscan Multiplex Plus, Airyscan Joint Deconvolution, LSM plus, Correlative Array Tomography (CAT), Spectral RICS, FCS/FCCS, Dynamics Profiler, Smart Acquisition

Funding

Please acknowledge the use of the instrument in publications the following way:

“We would like to thank the Microscopy Core Facility of the Medical Faculty at the University of Bonn for providing support and instrumentation funded by the Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 534092360”