Zeiss CellDiscoverer 7 high-content screening microscope

Location: BMZ 2, 2G.303

Technical Information:

Objectives

Magnification Type NA Immersion
5X Plan-Apochromat 0.35 dry
20X Plan-Apochromat 0.70 autocorr dry
20X Plan-Apochromat 0.95 autocorr dry
50X Plan-Apochromat 1.20 autocorr water

Afocal magnification changer (0.5X / 1.0X / 2.0X) is available for all objectives.

The 50X objective is equipped with automated water dispenser.

 

Optical setup

Light sources:

LED Set 01: 385, 470, 567 and 625 nm

LED Set 02: 420, 511 and 590 nm

Dichroic mirrors:

Quad-band (Zeiss, set 90 HE)

Tri-band (Zeiss, set 91 HE)

Emission filters:

Quad-band (Zeiss, set 90 HE)

Tri-band (Zeiss, set 91 HE)

CFP, 465/30 (AHF, F39-465)

GFP, 524/24 (AHF, F39-527)

YFP, 555/25 (AHF, F49-555)

RFP, 590/20 (AHF, F37-592)

 

The multi-band setup:

Color BFP CFP GFP YFP RFP AF594 AF647
Excitation 385 420 470 511 567 590 625
Dichroic 405 450 493 538 575 610 653
Emission 425/30 467/24 514/30 555/25 592/25 687/145 709/100

 

Camera

Axiocam 506m

 

Accessories

Environmental chamber

Various sample holders

Plate loader with 2×12 plate carousel

 

Software

ZEN 2.6 Pro

Modules: Tiles&Positions, Autofocus, Experimental Designer, Image Analysis, Advanced Processing, Measurement, Physiological package, Macro Environment

 

Funding

Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388158066