Zeiss Axio Scan.Z1 Slide Scanner

Future location: Building 82, UG1 056

Technical information:

Objectives

Magnification Type NA Immersion WD (mm) CVS (mm) #
5x Fluar 0.25 dry 12.5 0.17 420130-9900-000
10x PlanApochromat 0.45 dry 2.1 0.17 420640-9900-000
20x PlanNeofluar 0.50 dry 2.0 0.17 420350-9900-000
20x PlanApochromat 0.8 dry 0.55 0.17 420650-9902-000
40x PlanApochromat 0.95 dry 0.25 0.17 420660-9970-000

Requirements

Standard microscopy slides (76 x 26 mm) with rectangular coverslips (50 x 24 mm) [max. 100 slides capacity]

or: big microscopy slides (76 x 52 mm) with rectangular coverslips (75 x 50 mm) [max. two slides capacity]

Coverslip thickness 0.17 mm (high precision coverslips are recommended)

Bottom of microscopy slide has to be perfectly flat (e.g. free of any tape or label).

No cracks in microscopy slides or coverslips.

Coverslips must not protrude the microscopy slide.

Filters

Zeiss filter sets 90 HE LED, 91 HE LED, 110 HE LED for:

DAPI, CFP, FITC, YFP, TRITC, mCherry, Cy5 and Cy7

Cameras

Hitachi HV-F202SCL (color camera)

Hamamatsu Orca Flash 4.0 V3 (monochrome camera)

Light source

Colibri 7 (385 nm, 430 nm, 475 nm, 511 nm, 555 nm, 590 nm, 630 nm)

Accessories

Magazines for 100 pcs of 76 x 26 mm glass slides or 2 pcs of 76 x 52 mm glas slides.

Software

Zeiss Zen 2.x

Funding

Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388168919