VisiScope CSU-W1 with VS-Homogenizer Spinning Disk Microscope

Location: Building 82, UG1 056

Technical information:

Objectives

Mag. Type NA Immersion WD (mm) Coverslip (µm)
5x PlanNeofluar 0.16 dry 18.5 0.17
10x PlanApochromat 0.45 dry 2 0.17
20x PlanApochromat 0.8 dry 0.55 0.17
40x C-Apochromat 1.2 water ~ 0.28 0.14 – 0.19
63x C-Apochromat 1.2 water ~ 0.28 0.14 – 0.19
63x C-Apochromat 1.4 oil 0.14 0.17

Spinning disk unit

Yokogawa CSU-W1 with 25 µm and 50 µm pinhole disk

Laser lines

405 nm, 488 nm, 561 nm, 640 nm

Filters

DAPI, GFP, mCherry, Cy5

Cameras

Two pco.edge sCMOS cameras

Accessories

VS-Homogenizer for a flat intensity profile in the field of view

Definite Focus.2 for stabilized z-focus

CO2 (0-15%) and temperature (25-60°C) incubator system

Automated water immersion dispenser (for 40x and 63x water)

Piezo z-stage

Microscope stand

Zeiss Axio Observer

Software

VisiView Software

Funding

Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388169927