Leica SP5 AOBS with SMD confocal microscope

Location: BMZ 2, 2OG.303

Technical information:

Objectives

Magnification Type NA Immersion
10X HCPL Fluotar 0.30 dry
20X HCX PL APO 0.70 corr any
63X HCX PL APO 1.20 corr water
63X HCX PL APO 1.40 oil

Lasers

Argon, multiline (458, 476, 488, 496 and 514 nm)

DPSS (561 nm)

HeNe (633 nm)

Accessories

environmental chamber

CO2-incubation

automated water-immersion pump

Software

LAS AF v2.7.3

Wizards: FRAP, FRET, FCS, FLIM, MatrixScreener

SMD unit

The SMD (Single Molecule Detection) unit contains adjustable-frequency pulsed-lasers and sophisticated single-photon counting detectors for lifetime imaging (FLIM) and correlation spectroscopy (FCS).

PicoQuant hardware and software components

Lasers: 405, 470 and 640 nm

Detectors: 2 x SPAD

Filters: CFP / YFP, GFP / AF647, neutral split, polarization split

Funding

Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 169331223