Location: Anatomy Building, Untergeschloss, Nussalle, Poppelsdorf Campus
Technical information:
Objectives
| Magnification | Type | NA | Immersion |
|---|---|---|---|
| 10X | HCPL Fluotar | 0.30 | dry |
| 20X | HCX PL APO | 0.70 corr | any |
| 63X | HCX PL APO | 1.20 corr | water |
| 63X | HCX PL APO | 1.40 | oil |
Lasers
Argon, multiline (458, 476, 488, 496 and 514 nm)
DPSS (561 nm)
HeNe (633 nm)
Accessories
environmental chamber
CO2-incubation
automated water-immersion pump
Software
LAS AF v2.7.3
Wizards: FRAP, FRET, FCS, FLIM, MatrixScreener
SMD unit
The SMD (Single Molecule Detection) unit contains adjustable-frequency pulsed-lasers and sophisticated single-photon counting detectors for lifetime imaging (FLIM) and correlation spectroscopy (FCS).
PicoQuant hardware and software components
Lasers: 405, 470 and 640 nm
Detectors: 2 x SPAD
Filters: CFP / YFP, GFP / AF647, neutral split, polarization split
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 169331223
