Future location: Building 82, UG1 056
Technical information:
Objectives
| Magnification | Type | NA | Immersion | WD (mm) | CVS (mm) | # |
| 5x | Fluar | 0.25 | dry | 12.5 | 0.17 | 420130-9900-000 |
| 10x | PlanApochromat | 0.45 | dry | 2.1 | 0.17 | 420640-9900-000 |
| 20x | PlanNeofluar | 0.50 | dry | 2.0 | 0.17 | 420350-9900-000 |
| 20x | PlanApochromat | 0.8 | dry | 0.55 | 0.17 | 420650-9902-000 |
| 40x | PlanApochromat | 0.95 | dry | 0.25 | 0.17 | 420660-9970-000 |
Requirements
Standard microscopy slides (76 x 26 mm) with rectangular coverslips (50 x 24 mm) [max. 100 slides capacity]
or: big microscopy slides (76 x 52 mm) with rectangular coverslips (75 x 50 mm) [max. two slides capacity]
Coverslip thickness 0.17 mm (high precision coverslips are recommended)
Bottom of microscopy slide has to be perfectly flat (e.g. free of any tape or label).
No cracks in microscopy slides or coverslips.
Coverslips must not protrude the microscopy slide.
Filters
Zeiss filter sets:
90 HE LED (QBS 405 + 493 + 575 + 653 // QBP 425/30+514/30+592/30+709/100) e.g. for DAPI, FITX, TRITC, Cy5
91 HE LED (TBS 450+538+610 // TBP 467/24+555/25+687/145) e.g. for CFP, YFP, mCherry
110 HE LED (QBS 405 + 493 + 611 + 762 // QBP 425/30 + 524/51 + 634/38 + 785/38) e.g. for DAPI, FITC, mCherry, Cy7
Cameras
Hitachi HV-F202SCL (color camera)
Hamamatsu Orca Flash 4.0 V3 (monochrome camera)
Light source
Colibri 7 (385 nm, 430 nm, 475 nm, 511 nm, 555 nm, (590 nm,) 630 nm)
Attention: 590 nm cannot be combined with 555 nm and 630 nm
Accessories
Magazines for 100 pcs of 76 x 26 mm glass slides or 2 pcs of 76 x 52 mm glas slides.
Software
Zeiss Zen 2.x
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388168919
