Location: BMZ 2, 2OG.303
Technical information:
Objectives
| Magnification | Type | NA | Immersion |
|---|---|---|---|
| 10X | HCPL Fluotar | 0.30 | dry |
| 20X | HC PL APO CS2 | 0.75 corr | any |
| 40X | HC PL APO CS2 | 1.10 corr | water |
| 63X | HC PL APO CS2 | 1.20 corr | water |
| 63X | HC PL APO CS2 | 1.40 | oil |
Lasers
Diode (405 nm)
Argon, multiline (458, 476, 488, 496 and 514 nm)
DPSS (561 nm)
HeNe (633 nm)
Accessories
4 x HyD detectors for the best signal-to-noise ratio
Resonance scanner (8 kHz) for fast live imaging with minimal photo-toxicity
Environmental chamber
CO2-incubation
Software
LAS X v3.5.5
Wizards: FRAP, FRET, Lightning, Live Data Mode, Dye Finder, 3D Visualization, Navigator
Lightning deconvolution software
The Lightning module automatically detects the finest structures and details. The key is an adaptive process for extraction of hidden information in the image. Unlike traditional deconvolution techniques, that use a global set of parameters for the full image, Lightning calculates an appropriate set of parameters for each voxel to uncover every detail with the highest fidelity.
The Lightning detection concept extends the confocal microscope by simultaneous multi-color super-resolution imaging at the full original speed.
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388159768
