Dear users of the Core Facilities, Dear Colleagues, core facilites are a place to share instrumentation, spread information and support scienctists. People have to meet. But in order to fulfill our part in reducing the impact and spread of the novel coronavirus, the core facilities have taken some definite decisions to reduce service and the…
The European Journal of Immunology has published the second edition of the very comprehensive guide to flow cytometry and cell sorting in immunological studies. And we contributed to that edition. Here is the information from the abstract: “These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry…
In 2018 the Flow Cytometry Core Facility was able to acquire five new instruments. A FACSAria Fusion cell sorter, a second LSRFortessa cell analyzer and an ImageStream image cytometer were granted by the DFG through a large device application. (“Forschungsgroßgeräte” nach Art.91b GG) Special thanks goes to the excellence cluster “ImmunoSensation”and to the SFB TR57 . A…
Recent reports suggest that somatic cell-derived induced neurons (iNs), but not induced pluripotent stem cell (iPSC)-derived neurons largely preserve age-associated traits such as age-specific DNA methylation patterns, transcriptomic aging signatures and nuclear lamina-associated changes. Michael Peitz, manager of the Cell Programming Core Facility, co-directed a study which adressed a key question: Are age-associated epigenetic and transcriptional…
The European Journal of Immunology has published a very comprehensive guide to flow cytometry in it’s October issue in which we were involved. 231 authors: experts in cytometry & immunology 214 pages 54 sections including: ◦ equipment set-up, data acquisition & handling ◦ barcoding, CyTOF, combinatorial & imaging cytometry ◦ complete immune cell cytometric …
Dowling-Degos disease is a hereditary pigmentation disorder that generally progresses harmlessly. However, some of those affected also develop severe skin inflammation. An international team of researchers under the leadership of the University of Bonn has now found a cause for this link. Their knowledge comes thanks to an animal that is known among aquarium owners…
The Journal of Biological Chemistry, 291, 103-109. The mechanisms of NLRP3 activation are still poorly understood. Jonathan Schmid-Burgk and colleagues present new data on the identification of NEK7, which specifically functions upstream of NLRP3 activation. NEK7 was identified in an unbiased genetic screening approach, which employed the CRISPR technology to identify macrophages that were rendered…
Original published on Phys.org (Phys.org)—A team of researchers with the University of California has learned more about the process that is involved during CRISPR/Cas9 gene splicing by using a crystallization technique to get a closer look. In their paper published in the journal Science, the team describes their study of the way three-stranded structures known…
This would be the perfect time to ask something like ” how old is this flow cytometry stuff”. This month sees the 50 years anniversary of the first publication on cell sorting by Mack Fulwyler (Science 1965) and a first description of a Spectrophotometer for ultrarapid cell analysis by Louis Kamentsky and Myron Melamed (Science…
Originally posted by Rachel Walker on BitesizeBio The helpful people who work in your flow core are an amazing resource of information for your experiments and are the people with the power to get experiments done. However, you need to be nice to these powerful people, so here are a few handy hints: Its all…