Due to ongoing technical advancements Next Generation Sequencing (NGS) is widely applied in many research projects. But, despite decreasing sequencing costs during the last years, the access to this technology is made difficult as investment in and maintenance of NGS systems as well as the establishment of research specific applications remains expensive and time consuming. Therefore, the NGS Core Facility, which is allocated at the Institute of Human Genetics in Bonn, was founded in 2016 and has been become the operating site of the West German Genome Center (WGGC) in Bonn in 2019. Its mission is to provide the research community with access to state-of-the art sequencing technologies and to help facilitate cutting-edge-omics research. This service is all-encompassing as it is intended to cover all phases of your project: We offer rapid high-throughput sequencing services, consultation and expert assistance in strategic planning, sample preparation and method development. Therefore, researchers are encouraged to contact the NGS Core Facility for consultation prior to sequencing projects, which will allow us to provide initial support in study design and therefore optimize the performance of the NGS experiments.
Typical sequencing protocols are:
- Whole genome sequencing (human, plant, animal, microbe, virus)
- Exome sequencing
- Transcriptome sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling)
- Chromatin analysis (ChIP Seq, ATAC Seq)
- Metagenomics (16S rRNA or shotgun metagenomics)
- Targeted gene sequencing (amplicon or gene panel)
Upon sequencing completion, the data are primarily analysed (demultiplexing) and provided to the scientist per cloud-based download-link in standard format fastq. If a more complex statistical analysis is needed, we recommend to get in contact with the local core facility Bioinformatics.