As there are plenty of specific protocols for library preparation available we cannot provide a complete summary of costs calculations on the website. Therefore, this is just a brief overview for the most requested protocols. Please feel free to contact us for detailed information and current cost estimations for your sequencing project.
Please consider, that in case of placing an order costs for materials (kits, consumables, sequencing chemistry) and overheads will arise. Please make sure that you are allowed to pay these different types of costs from your specific account (PSP-Element). If you are not sure about that, do not hesitate to contact us.
3´-mRNA Sequencing (expression analysis):
This is an appropriate protocol for comparing varying expression levels in different sample groups (like treated vs. untreated, healthy vs. affected, etc.). The libraries are prepared by using the QuantSeq FWD 3´-mRNA-Seq Kit from Lexogen. The sequencing is done on the NovaSeq 6000 with a read length of 1x100bp and in most cases an average of 10 M raw reads are generated per sample. The shown price includes the costs for RNA QC, library preparation and sequencing on a NovaSeq S1 flow cell. If the RNA was extracted from whole-blood samples, it is recommended to reduce specifically the proportion of globin transcripts.
Whole Exome Sequencing
Whole exome sequencing enables deep-sequencing of coding sequences of the genome. The aim of this approach is to detect genetic variants which could have an effect on protein sequence and function. The libraries are prepared by using the Human Comprehensive Exome Panel from Twist Bioscience. The sequencing is done on the NovaSeq 6000 with a read length of 2x100bp and in most cases an average of 6 or 12 Gb are generated per sample. The shown price includes the costs for DNA QC, library preparation and sequencing on a NovaSeq S1 flow cell.
Whole Genome Sequencing
Whole genome sequencing enables deep-sequencing of both coding and non-coding sequences of the genome. The aim of this approach is either to detect genetic variants in a known genome which could have an effect on protein sequence and function or to build de novo assemblies of unknown/poorly known genomes. The libraries are prepared by NEB Ultra II DF DNA PCR-free Library Prep. The sequencing is performed on a NovaSeq X plus with a read length of 2x150bp and in most cases an average base coverage of 30x is generated. The shown price includes the costs for DNA QC, library preparation and sequencing on a NovaSeq X plus 10B flow cell.
