Injection microscope
Our facility operates an inverted phase-contrast microscope equipped with micromanipulators and a Piezo element. This setup applies for a variety of mouse embryo micromanipulation techniques, including pronuclear and intracytoplasmic injections into oocytes as well as blastocyst injections.
Further we are equipped with an electroporation device, allowing for a fast and high-throughput manipulation of oocytes for an efficient and cost-effective generation of CRISPR/Cas9 gene-edited mice.
Micromanipulated embryos are transferred into pseudopregant foster mice, either by oviduct transfer of 2-cell stage embryos or by in utero transfer of blastocysts. Foster mice are kept in the animal house under specific-pathogen-free (SPF) conditions in individually ventilated cages (IVCs) and are checked daily for their health.
