The gene-editing core facilities offer services for the generation of gene-edited and transgenic mice. In the past decade the discovery of the CRISPR/Cas9 system has revolutionized biomedical research.
The application of this versatile genome-editing tool has also accelerated the generation and establishment of genetically modified mouse models. Gene knockouts, conditional mice as well as insertions of small tags or point mutations can be achieved without elaborate and time consuming cloning of targeting vectors and subsequent recombination in murine embryonic stem cells.
Thus, the core facilities aim to utilize CRISPR/Cas9-mediated approaches for the generation of genetically modified mice whenever possible. Furthermore, we are actively working on improvements and refinements of the editing technologies, considering latest publications and methodologies in the field.