FACSymphony A5

Location

Campus Poppelsdorf, LIMES Institute, Raum I-227

Description

The BD FACSymphony offers high-parameter single cell acquisition at high speed. Currently, the system is capable of recording 30 parameters (28 fluorescence channels + light scatter channels) at up to 40,000 events per second, and has the potential to be upgraded to 50 parameters.

The high number of acquisition parameters and the high resolution make the FACSymphony the ideal instrument for the identification and analysis of rare cell types and in-depth or broad phenotyping of single cell events at high-throughput.

A High Throughput Sampler (HTS) for 96-well plates can be used for efficient high-throughput screening approaches but requires an additional introduction before usage.

Book in PPMS or Request a training

Contact persons

Prof. Elvira Mass

Available Configuration:

Laser	Position	Filter	LP	Fluorochrome
355nm	A	810/40	770	BUV805
UV	B	735/30	690	BUV737
60-100mW	C	670/25	630	BUV661
	D	605/20	595	BUV615
	E	580/20	550	BUV653
	F	515/30	450	BUV496 (DAPI)
	G	379/28		BUV395 (Hoechst)

405nm	A	780/60	750	BV786
Violet	B	750/30	735	BV750
200mW	C	710/20	685	BV711
	D	677/20	635	BV650
	E	605/40	595	BV570
	F	586/15	550	BV510/BV480
	G	525/50	505	BV421
	H	431/23	570LP

488nm	A	780/60	750	BB790-P
Blue	B	710/50	685	BB700/PerCp-Cy55
200mW	C	670/30	635	BB660-P (PerCP)
	D	610/20	600	BB630-P
	E	530/30	505	BB515/FITC
	F	488/10

561nm	A	780/60	750	Pe-Cy7
Yellow-Green	B	710/50	685
	C	670/30	635	Pe-Cy5
	D	610/20	600	Pe-CF594(PE-Dazzle)
	E	586/15	570	PE

637nm	A	780/60	750	APC-Cy7
Red	B	730/45	690	C-R700/Alexa Fluor 700
140mv	C	670/14	RG665	APC/AF647