Flow Cytometry Day 2024
All-day theoretical lectures on flow cytometry for beginners and advanced students.
Invited speaker: Thomas Bauer-Jazayeri
Program:
10.00 – 10.30 Introduction: Flow Cytometry Core Facility Bonn by Andreas Dolf
Learn more about your Core Facility the Team and their expertise as well as about the available instrumentation and coupled analysis possibilities.
10.30 – 12.00 Flow Cytometry:
What’s in the Box; Principals and technology of Flow Cytometry and how this is relevant to your application, experimental design and analysis. This includes the instrumental set-up, optics, filters, staining, nomenclature and troubleshooting. Furthermore, we will discuss the impact and requirements of instrument settings for required sensitivity for your experiment.
12.00 – 12.45 Lunchbreak
12.45 – 14.00 How to Stain what:
Learn more about the properties of the different available dyes and how to use this information for your Panel Design in Multicolor Flow Cytometry.
14.00 – 14.15 Break
14.15 – 15.15 Data Acquisition:
Relevant and required tools to optimize quality, accuracy, precision, and sensitivity of your analysis towards your experimental requirements to receive valuable and relevant data.
15.15 – 15.30 Break
15.30 – 16.00 Antibodies as Biomarkers: How to reliably detect multiplexed proteomic changes
The individual Immune-Profile represents an excellent biomarker and is used as diagnostic, prognostic or predictive tool and is already used in diseases like Cancer, Autoimmunity, Neurology or Infectious disease. Learn how to take advantage of antibody profiling and how this can be realized with high-precision, high-reproducibility and high-sensitivity in a biologically relevant form.
16.00 – 16.30 Spectral Flow Cytometry (Andreas Dolf)
Spectral Flow Cytometry is a technique based on conventional Flow Cytometry where a spectrograph and multichannel detector is substituted for the traditional mirrors, optical filters and photomultiplier tubes in conventional systems. Learn more about this technology and its applications and benefits.
THIS SESSION IS THOUGHT TO BE INTERACTIVE: BE AN ACTIVE PART OF IT!