Services

The Core Facility „Transgenics“ (CFT) provides services for generation, rederivation and preserving genetically modified mice. The Core Facility provides its services at net cost price for internal users. Advice is provided at no charge!

You need help or have got a question? The CFT can also help you to find the right Cre mouse line for your conditional knock out project.
Email the CFT: CFT@uni-bonn.de

Do you already have a project with us?  Follow your project via OpenProject.

We provide the following services:

Standard DNA microinjection (pronucleus)

CRISPR/Cas9 microinjection

Stem cell injection (morulae or blastocysts)

Rederivation

Embryos

Sperm

Cryopreservation

Embryos

Sperm

      Sanitation of mouse lines

Sanitation of mouse lines is now under the responcibilities of the House of Experimental Therapy (HET).

For more details please visit http://www.ukb.intern/C125696000418A5A/direct/haus-fuer-experimentelle-therapie and contact the HETs leaders.

      Reactivation of mouse lines

In case a mouse line has quit its reproductive performance the CFT can help to reinstall the line via in-vitro fertilization. This also applies for non-productive founder male mice.

      Biomolecular services

Generation of targeting constructs

BAC isolation and recombination